Journal: Structure (London, England : 1993)

Article Title: Structural and Functional Analysis of E. coli Cyclopropane Fatty Acid Synthase

doi: 10.1016/j.str.2018.06.008

Figure Lengend Snippet: Data software and availability

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains E. coli T7 Express New England Biolabs C2566I Chemicals, Peptides, and Recombinant Proteins Trypsin Sigma-Aldrich T8003 SigmaFAST protease inhibitor cocktail, EDTA-free Sigma-Aldrich S8830 Guanidine hydrochloride Sigma-Aldrich 50933 E. coli polar lipid extract Avanti Polar Lipids, Inc 100600 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB) Thermo Fisher Scientific 22582 DyLight ™ 488 Maleimide Thermo Fisher Scientific 46602 DyLight ™ 650 Maleimide Thermo Fisher Scientific 62295 Deposited Data Crystal structure of E. coli CFA synthase This paper PDB: 6BQC Crystal structure of mycolic acid cyclopropane synthase CmaA2 Huang et al., 2002 PDB: 1KPI Crystal Structure of Mycolic Acid Cyclopropane Synthase PcaA Huang et al., 2002 PDB: 1L1E Crystal Structure of mycolic acid cyclopropane synthase CmaA1 Huang et al., 2002 PDB: 1KPH Crystal structure of a novel protein from Mycobacterium smegmatis Shahine et al., 2014 PDB: 4NSS Protein sequence of cyclopropane synthase from Sterculia foetida Bao et al., 2002 GenBank: {"type":"entrez-protein","attrs":{"text":"AAM33848.1","term_id":"21069167","term_text":"AAM33848.1"}} AAM33848.1 Oligonucleotides See Table S1 Recombinant DNA His6-TEV-CFA synthase Cloned from genomic DNA Gene ID 944811 His7-SUMO-CFA synthase Cloned from genomic DNA Gene ID 944811 His6-TEV-AdoHcy nucleosidase Cloned from genomic DNA Gene ID 948542 His6-TEV-LuxS Cloned from genomic DNA Gene ID 947168 Software and Algorithms SEDFIT Brown and Schuck, 2006 14.7g SEDNTERP John Philo 2 PHENIX Adams et al., 2010 1.12_2829 PHASER McCoy et al., 2007 2.8.2 HKL2000 Otwinowski and Minor, 1997 716.1 Open in a separate window Data software and availability

Techniques: Software, Recombinant, Protease Inhibitor, Sequencing, Clone Assay